Setting up cameras and filters spectra

Setting up cameras and filters spectra


Some analysis functions (e.g., run_HemoCompute) in the toolbox need to use the optical parameters from the acquisition system, such as the spectral profiles of filters, illumination sources, and cameras. This information is stored in .mat files inside the folder .../Umit/SystemOpticalParams and can be updated as needed. Here is a description of each file:

SysSpect.mat

Contains a list of all available spectral profiles from filters and cameras. The spectrum of each item consists of a vector with a length of 301 samples. The data is normalized from 0 to 1, and the wavelength ranges from 400 to 700 nm. Here is an example of the spectral profile of a band-pass filter at 472 nm (FF0247230):

FF02-472/30 Spectral Profile

You can add your own profile to this list, if your filter/camera/illumination profile is not listed.

FilterSets.mat

This file contains a list of filter combinations (or sets) used. Each variable is a Matlab structure that contains the fields Excitation and Emission. The value of each field is the name of the spectral profile stored in the SysSpect.mat file. You can add your own filter sets to this file, provided that the spectral profile is listed in the SysSpect.mat file.

CameraSpect.mat

This file contains a list of camera models and their corresponding spectral profiles in the SysSpect.mat file. The existing cameras are the following:

  • CS2100M
  • D1024
  • D1312

As with the FilterSets.mat file, you can add your own camera model with the corresponding spectral profile.



Back to top

Copyright © LabeoTech 2024. This work is licensed under the GNU General Public License, Version 3.0.