Exploring temporal traces
Compare the time course of a selected pixel and visible ROIs without first exporting data. Open Y-X-T data, then create or load at least one ROI.
- Click a pixel to update the black temporal profile.
- Make one or more ROIs visible in the ROI table. A colored mean trace appears for each visible ROI.
- Move the frame slider and confirm that the vertical time marker and current-frame ROI statistics update.
- Switch to Event mode to compare a condition, repetition, or average when event information exists.
- Choose ROIs > Export ROI data to export spatial measurements, continuous time vectors, or event-aligned time vectors.
The visible red ROI appears in the table and contributes the matching red trace to the temporal plot.
What is saved?
Trace calculation is read-only and may use a disk cache. CSV export is an option in the ROIs tab under Export ROI data; see Save, load, import, and export. The export does not change the ROI file or imaging data.
For calculations intended for later statistics, run getDataFromROI in a pipeline. It accepts .dat or .umt data with Y-X axes and returns a .umt file with the data organized by ROI. Event response features require the ordered workflow split_data_by_event (which writes an event-split .dat file) → getDataFromROI with spatial aggregation enabled → calculateResponseFeatures (which takes the .umt file produced by getDataFromROI). To load and use these .umt files in MATLAB, see Organization of .umt files.